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The blot has been probéd with Anti-Phóspho-GSK3 alphabeta (Sér9, Ser21) Bunny Monoclonal Antibody (Product Mother5-15023, 1:500 dilution) and recognized by chemiluminescence using Goat anti-Rábbit IgG (HL) SupercIonal Extra Antibody, HRP conjugate (Product A27036, 0.25 gmL, 1:4000 dilution).A 51 kDa music group related to Phospho-GSK3 alpha was recognized in untreated lysate and decreased upon SB216763 treatment.Western mark analysis making use of Phospho GSK3 leader beta (ser 9Ser21) antibody (Product MA5-15023), shows decrease in expression of Phospho GSK3 alpha (ser 21) upon treatment with SB216763.
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